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Figure 2 | SpringerPlus

Figure 2

From: Synergistic antitumor effects of S-1 with eribulin in vitro and in vivo for triple-negative breast cancer cell lines

Figure 2

TGF-β induces EMT in MDA-MB-231 cells after 5 days of exposure and reduced the sensitivity to 5-FU. (a) Morphological changes of MDA-MB-231 cells after exposure to 2.5 ng/mL of TGF-β. (b) The mRNA expression levels of E-cadherin, N-cadherin, vimentin and Snail2 were determined using real-time RT-PCR. GAPDH was used to normalize the expression levels. The data shown represent the average ± SD of three independent experiments. *P < 0.05 versus control. (c) Western blotting analysis for E-cadherin, N-cadherin, vimentin, and Snail2. β-actin was used as an internal control. (d) Immunofluorescence staining of E-cadherin and vimentin. The nucleus (blue) was stained with DAPI. (e) Cellular growth inhibition curves of 5-FU in MDA-MB-231 cells with or without TGF-β exposure.

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