Figure 1

Alternative splicing of intron 4 mini-gene containing the C/C or T/T genotype. A: human TCF7L2 gene, exons are represented by vertical lines. Grey boxes represent the parts of the gene that have been cloned to create the mini-gene B: amplification of four DNA segments (ex4: segment containing exon 4 and part of the beginning of intron 4; in4C: part of intron 4 containing the SNP with the C/C genotype; in4T: part of intron 4 containing the SNP with the T/T genotype; ex5: segment containing the end of intron 4 and exon 5). C: Two pcDNA3 plasmids were obtained with either the C/C (ex4-in4C-ex5) or the T/T (ex4-in4T-ex5) genotype. D: 72 h after MIN6 cells were transfected with plasmids, RT-PCR and an agarose gel were run, n = 4 independent experiments.