Table 2 Effect of plant growth regulators for callus induction, their further proliferation into multiple shoots through leaf explants
Plant growth regulators (mg l-1) | Leaf derived calli | ||||
|---|---|---|---|---|---|
BAP | NAA | 2,4-D | IBA | % response | Number of shoots/explant*Mean ± S.E. t0.05 |
0.5 | 20 | 2.25±0.10 | |||
1.0 | 35 | 2.88±0.50 | |||
1.5 | 40 | 3.15±0.22 | |||
2.0 | 48 | 6.11±1.50 | |||
2.5 | 46 | 5.50±0.40 | |||
3.0 | 30 | 4.90±0.10 | |||
3.5 | 23 | 3.10±0.20 | |||
2.0 | 0.5 | 19 | 2.20±0.10 | ||
2.0 | 1.0 | 22 | 2.45±0.15 | ||
2.0 | 2.0 | 35 | 2.75±0.25 | ||
2.0 | 3.0 | 37 | 3.55±1.50 | ||
2.0 | 4.0 | 28 | 3.40±0.40 | ||
2.0 | 5.0 | 16 | 2.90±0.10 | ||
2.0 | 0.5 | 9 | 0.90±1.05 | ||
2.0 | 1.0 | 12 | 1.15±0.22 | ||
2.0 | 2.0 | 20 | 1.91±0.54 | ||
2.0 | 3.0 | 31 | 3.15±0.42 | ||
2.0 | 4.0 | 24 | 2.56±0.56 | ||
2.0 | 5.0 | 21 | 2.14±0.15 | ||
2.0 | 0.025 | 80 | 11.14±0.90 | ||
2.0 | 0.5 | 95 | 12.00±1.50 | ||
2.0 | 1.0 | 75 | 9.28±1.00 | ||
2.0 | 2.0 | 68 | 8.75±0.80 | ||
2.0 | 3.0 | 55 | 6.45±0.25 | ||