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Table 1 Details of primers, bands and polymorphism in T. indica revealed by 6 primers used in ISSR–PCR

From: Assessment of clonal fidelity of Tylophora indica (Burm. f.) Merrill “in vitro” plantlets by ISSR molecular markers

Primers

Sequences

Total No. of bands (a)

Total No. of Polymorphic bands (b)

Polymorphism (b/a*100)

Total No. of monomorphic bands (c)

Monomorphism (c/a*100)

Average PIC

 

(5’→3’)

      

807

AGAGAGAGAGAGAGAGT

8

7

87.50

1

12.50

0.5

812

GAGAGAGAGAGAGAGAA

16

6

37.500

10

62.50

0.82812

825

ACACACACACACACACT

12

4

33.333

8

66.66

0.875

827

ACACACACACACACACC

11

11

100.000

0

0

0.56818

840

GAGAGAGAGAGAGAGAYT

5

3

60.000

2

40

0.7

846

CACACACACACACACART

19

10

52.632

9

47.36

0.71052

Total

 

71

41

57.74

30

42.26

0.57

  1. *= Multiply.