Figure 3
Changes in epidermal cell morphologies following 24 hrs of imbibition. a-j, in situ PODa, AEC + H2O2; a-b, view of scutellum epidermis and parenchyma; c-i, transformation of epidermis, presence of PODa in apoplast, and regional alterations of FL; j, area of scutellum where 3 regions are observed, showing asynchrony of transformation process for epidermis and FL; the section was incubated with AEC without H2O2, showing sites of production of endogenous peroxides. Abbreviations: ep, epidermal cells; fl, fibrous layer; pc, parenchyma cells; se, central starchy endosperm. Symbols: ▲, epidermal cells in the process of transformation; ↑, structural changes in fibrous layer; ✦, apoplastic PODa. Bar: a, c-i: 50 μm; b: 125 μm; j: 500 μm.