Figure 2

Diffusion through fibrous layer. Dry grains were perforated through the embryo or endosperm, and the diffusion of aqueous or ethanol dyes was assessed; a, grain without stain; b-e, diffusion of I₂-KI; f-j, diffusion of Brilliant Green; b, grain without perforation; c, grain perforated through both compartments; d and f, perforation through embryo; e and g, perforation through endosperm; h and i, grains perforated through endosperm, and h, treated with acetone, or i, treated with chloroform; j, grains perforated through embryo and treated with acetone:chloroform. Abbreviations: WS, without stain; WP, without perforation; FP, full perforation; Emb ChP, embryo chamber perforation; End ChP, endosperm chamber perforation.