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Figure 3 | SpringerPlus

Figure 3

From: Cloning and expression of Aspergillus flavus urate oxidase in Pichia pastoris

Figure 3

Western blot analysis on supernatant of GS115 transformant culture media. A: Western blotting using anti-His tag antibody. Lane 1: Concentrated supernatant of GS115 positive transformant culture media after 4 days of induction. Lane 2: Concentrated supernatant of empty vector transformant. Lane 3: 18 kDa his-tagged protein as a positive control. B: Western blotting using anti-rasburacase antibody. Lane 1: positive control (commercial rasburicase), Lane 2: Negative control (Concentrated supernatant of empty vector transformant) and Lane3 Concentrated supernatant of GS115 positive transformant. Black arrows show the expressed urate oxidase.

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