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Figure 1 | SpringerPlus

Figure 1

From: Coregulation of FANCA and BRCA1 in human cells

Figure 1

Differential cell cycle regulation of FA genes in human T98G cells. (a-left panel) FACS analysis of synchronized cells, the 0, 8, 12, 16, 18, 20, 22, 24, 28, and 32 hr time points are shown; S phase is most pronounced between 18–22 hrs. Data of FACS represents one representative synchronization (a-right panel) Control experiment: The mRNA levels of CCNE2 during the cell cycle are shown. Quantitative RT-PCR was performed on total RNA samples from different time points and mean fold changes (MFC) were calculated relative to time point zero. Data represents the average mean fold change of 2–3 independent synchronization each in duplicate qPCR measurement (except for time points: 28 and 32) (b-g) Relative gene expression during the cell cycle for genes encoding in the FA/BRCA-pathway.

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