Oxygen-limited cellobiose fermentation and the characterization of the cellobiase of an industrial Dekkera/Brettanomyces bruxellensis strain

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Table 4 Physiological parameters at the end of fermentation by Dekkera bruxellensis strain GDB 248

Media^*	Sugar	Sugar supplied (g/L)	Residual sugar (g/L)	qS(-g/L.h)	Glycerol (g/L)	Acetate (g/L)	Ethanol (g/L)	P_max(g/L.h)	Fermentation efficiency (%)
SMSsuc	Sucrose	25.00	0.00	4.79	0.00	6.64(±1.08)^ab	5.76(±0.21)^a	0.24	42.85
SMScello	Cellobiose	20.50	0.00	1.26	0.00	10.87(±1.85)^a	3.72(±0.39)^b	0.15	33.71
SMScello/glu	Cellobiose	10.00	0.00	0.43	0.18(±0.04)^a	4.73(±0.89)^b	5.88(±0.49)^a	0.25	56.06
SMScello/glu	Glucose	10.00	0.00	2.89	0.18(±0.04)^a	4.73(±0.89)^b	5.88(±0.49)^a	0.25	56.06
SMSbag	Cellobiose	4.45	3.41(±0.30)^a	0.04	0.49 ±(0.04)^b	5.38(±0.24)^b	4.87(±0.40)^ab	0.10	53.95
	Glucose	17.58	0.95(±0.06)^b	0.18

*Synthetic YNB medium containing sucrose (SMSsuc), cellobiose (SMScello), a mixture of cellobiose and glucose (SMScello/glu) or acid pre-treated enzyme-hydrolyzed sugarcane bagasse (SMSbag). Rates of sugar consumption (qS) and maximal ethanol production (P_max) as well as the percentage of maximal theoretical yield were calculated. Average values (and standard deviation in parenthesis) were calculated from biological replicates with technical replicates for each sample. Similar superscript letters indicate no statistical difference according to ANOVA test (p < 0.05).