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Table 3 Effect of disaccharides on the activity of the purified cellobiase from Dekkera bruxellensis GDB 248

From: Oxygen-limited cellobiose fermentation and the characterization of the cellobiase of an industrial Dekkera/Brettanomyces bruxellensis strain

Substrate

Glucosyl link

Relative activity (%)

Inhibitory activity (%)a

Cellobiose

Glucose-β(1 → 4)β-Glucose

100.0

100.0

Maltose

Glucose-α(1 → 4)α-Glucose

27.7 ± 0,01

94.9

Sucrose

Glucose-α(1 → 2)β-Fructose

90.0 ± 0,03

95.2

pNPG

Glucose-β(1 → 4)β-phenyl

100.0

nab

pNPGAL

Galactose-β(1 → 4)β-phenyl

0.0

0.0

  1. aDissacharides were added to reactions with the chromogenic substrate pNPG.
  2. bnot applicable.