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Table 2 Summary of partial purification of cellobiase from Dekkera bruxellensis GDB 248 grown on cellobiose medium

From: Oxygen-limited cellobiose fermentation and the characterization of the cellobiase of an industrial Dekkera/Brettanomyces bruxellensis strain

Purification stepa

Protein concentration (mg/mL)

Specific activity (μmol EqGlucose/min.mgProtein)b

Purification factor

Cell-free extract

0.95

0.104

1.0

EF1c

1.19

0.113

1.09

EF2d

0.11

0.848

8.16

  1. aFive grams of yeast pellet was used for protein extraction and purification.
  2. bβ-Glucosidase activity was assay with 100 mM of p-nitrophenyl-β-D-glucopyranoside (pNPG) at 30°C.
  3. cActive fraction collected from molecular exclusion chromatography.
  4. dActive fraction collected from ion exchange chromatography.
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