Oxygen-limited cellobiose fermentation and the characterization of the cellobiase of an industrial Dekkera/Brettanomyces bruxellensis strain

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Table 1 Effect of different carbon source medium on enzyme activity in the crude cell-free extracts from Dekkera bruxellensis GDB 248

Sugar in the medium	Substrate of enzyme reaction	Enzyme assayed	Specific activity (U/mg protein)^a
Glucose	Sucrose	Invertase	0.998 ± 0.01
	Cellobiose	β-glucosidase	0.835 ± 0.02
	Maltose	α-glucosidase	0.000
Sucrose	Sucrose	Invertase	0.598 ± 0.01
	Cellobiose	β-glucosidase	0.024 ± 0.03
	Maltose	α-glucosidase	0.000
Cellobiose	Sucrose	Invertase	0.295 ± 0.01
	Cellobiose	β-glucosidase	0.460 ± 0.02
	Maltose	α-glucosidase	0.000

^aOne unit of enzyme activity (U) refers to the μmol of glucose released per minute at 30°C from the amount of protein in one gram of yeast cells.