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Table 3 Effects of different cations with the purified xylanase from A. pullulans CBS 135684 cultivated in basal medium containing 1% (w/v) corncob

From: Effect of polyols on thermostability of xylanase from a tropical isolate of Aureobasidium pullulans and its application in prebleaching of rice straw pulp

Additive

Relative enzyme activity (%)* at an additive concentration of:

 

1 mM

10 mM

None

100.0 ± 2.0abc, NS

100.0 ± 1.0C, NS

Fe2+

99.9 ± 4.5abc, 2

74.1 ± 1.2B, 1

Mg2+

105.9 ± 3.0c, 1

111.7 ± 5.1E, 2

Ca2+

112.4 ± 1.5d, NS

112.4 ± 2.8E, NS

Cu2+

95.5 ± 2.2a, 2

29.9 ± 2.7A, 1

Co2+

103.4 ± 4.1bc, 1

106.1 ± 3.5D, 2

Zn2+

99.2 ± 2.5ab, NS

98.4 ± 2.4C, NS

EDTA

100.9 ± 5.3abc, NS

99.6 ± 1.2C, NS

  1. The data were calculated from relative activity at different concentration of additives. The enzyme activity was assayed at 70°C for 30 min with 1% (w/v) beech wood xylan in 50 mM acetate buffer (pH 6.0).
  2. *One hundred percent activity corresponded to the activity of the enriched xylanase under standard assay conditions (without additive). Mean ± one standard deviation derived from three replicates (N = 3). Different superscript letter (a,b,c,A,B,C,D,E) in the same column indicated the values were significantly different (ANOVA and DMRT, P < 0.05) and different superscript number in the same row (1,2) indicated the values were significantly different (Student’s t-test, P < 0.05).
  3. NS = not significantly different.