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Table 2 Purification steps of xylanase isolated from A. pullulans CBS 135684 cultivated in basal medium containing 1% (w/v) corncob

From: Effect of polyols on thermostability of xylanase from a tropical isolate of Aureobasidium pullulans and its application in prebleaching of rice straw pulp

Purification steps

Total protein (mg)*

Total activity (U)a,*

Specific activity (U.mg-1)*

Purification (fold)*

Yield (%)*

Culture supernatant

2,030.00 ± 32.24

4,850.00 ± 36.42

2.39 ± 0.04

1.00

100.00

Ultrafiltration

1,376.00 ± 26.18

4,431.00 ± 27.31

3.22 ± 0.06

1.35

91.40

(NH4)2SO4 precipitation (50–80% saturation)

560.00 ± 1.24

2,440.00 ± 14.24

4.36 ± 0.02

1.81

50.30

DEAE-Sepharose

21.00 ± 1.04

834.00 ± 9.84

39.70 ± 1.76

16.60

17.20

Sephacryl S-100

16.00 ± 1.24

662.00 ± 10.46

41.40 ± 2.85

17.30

13.60

  1. a1 U = the amount of enzyme required to release 1 μmol xylose equivalent per min under the assay conditions.
  2. *Mean ± one standard deviation derived from three replicates (N = 3).
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