Identification of novel glycosyl hydrolases with cellulolytic activity against crystalline cellulose from metagenomic libraries constructed from bacterial enrichment cultures

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Table 1 Clones containing glycosyl hydrolase genes from metagenomic libraries constructed from different environmental samples

Library source	Screening number (Library size)	Active clone		Enzyme name	Sequence name (accession no.)
Soil	30,000 (130 Mb)	n.d.		-	-
Avicel 1st enrichment culture	25,000 (108 Mb)	Cellulase	p1a1	C1A1	1A1 (AB922008)
Avicel 1st enrichment culture	25,000 (108 Mb)	Cellulase	p1a2, p1a3, p1a4	C1A2	1A2 (AB922009)
Avicel 2nd enrichment culture	32,000 (138 Mb)	Cellulase	p2a1	C2A1	2A1 (AB922010)
		Cellulase	p2a2, p2a4	C1A2	1A2 (AB922009)
		Cellulase	p2a3	C2A3	2A3 (AB922011)
Pulp 1st enrichment culture	23,000 (100 Mb)	n.d.		-	-
Pulp 2nd enrichment culture	40,000 (172 Mb)	Cellulase	p2p3, p2p4, p2p4	C2P3	2P3 (AB922013)
Pulp 2nd enrichment culture	40,000 (172 Mb)	Xylanase	p2p1	X2P1	2P1 (AB922012)