From: Multiplex PCR assay for identification of commonly used disarmed Agrobacterium tumefaciens strains
A. tumefaciens Strain | Chromosome | Antibiotic gene on chromosome | Ti Plasmid | Antibiotic gene on plasmid | Opine | Target gene (Accession Number) (Nucleotide coordinates) | Primers name and sequence | Primer Tm (°C) | Product size (bp) | References |
---|---|---|---|---|---|---|---|---|---|---|
GV3101 | C58 | Rifampicin | Cured | - | Nopaline | GlyA gene (Accession no. EU334236.1) (1160791-1161213 nt) | C58 GlyA-F: 5′- CCACCACCACGACGCACAAGTCT -3′ C58 GlyA-R: 5′-TGCCGAGACGGACACCCGAC -3′ | 63.7 64.3 | 423 | (Holsters et al. 1980) |
C58C1 | C58 | - | Cured | - | Nopaline | (Deblaere et al. 1985) | ||||
EHA101 | C58 | Rifampicin | pEHA 101(pTiBo542ΔT-DNA) | Kanamycin | Succinamopine | (Hood et al. 1986) | ||||
EHA105 | C58 | Rifampicin | pEHA 105(pTiBo542ΔT-DNA) | - | Succinamopine | (Hood et al. 1993) | ||||
EHA101 | C58 | Rifampicin | pEHA 101(pTiBo542ΔT-DNA) | Kanamycin | Succinamopine | accF gene (Accession no. NC_010929) (237186-237952 nt) | pTiBo542-F: 5′-CCCGCTGAGAATGACGCCAA-3′ pTiBo452-R: 5′-CCTGCGACACATCGTTGCTGA-3′ | 60.4 60.2 | 766 | (Hood et al. 1986) |
EHA105 | C58 | Rifampicin | pEHA 101 (pTiBo542ΔT-DNA) | - | Succinamopine | (Hood et al. 1993) | ||||
EHA101 | C58 | Rifampicin | pEHA 101(pTiBo542ΔT-DNA) | Kanamycin | Succinamopine | nptI gene (Accession no. D85525.1) (929-357 nt) | nptI-F: 5′-CTGCGATTCCGACTCGTCCA-3′ nptI-R: 5′-CGGGCAATCAGGTGCGACA-3′ | 59.4 59.5 | 572 | (Hood et al. 1986) |
LBA4404 | Ach5 | Rifampicin | pAL4404 | Spectinomycin & streptomycin | Octopine | FtsZ gene (Accession no. EU334225.1) (48-416 nt) | Ach5FtsZ-F: 5′-GAACTTACAGGCGGGCTGGGT-3′ Ach5FtsZ-R: 5′-CGCCGTCTTCAGGGCACTTTCA-3′ | 62.0 62.1 | 369 | (Hoekema et al. 1983) |