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Table 1 The reactivity of various primer sets in multiplex PCR amplification to differentiate different Agrobacterium and E. coli strains

From: Multiplex PCR assay for identification of commonly used disarmed Agrobacterium tumefaciens strains

Primers pair set

Tm (°C) Used

 

Agrobacterium species

E. coli strains

Agrobacterium tumefaciens

Agrobacterium vitis

Agrobacterium rhizogenes

 

Strains

GV3101

C58C1

EHA105

EHA101

LBA4404

Ag57

Ag63

R3

DH5α

BL21

C58GlyA-F/R

61

 

+*

+

+

+

-*

-

-

-

-

-

pTiBo542-F/R

61

 

-

-

+

+

-

-

-

-

-

-

nptI-F/R

61

 

-

-

-

+

-

-

-

-

-

-

Ach5FtsZ-F/R

61

 

-

-

-

-

+

-

-

-

-

-

  1. *The reactivity of primer set with various Agrobacterium strains is indicated with (+) sign in case of positive PCR amplification while with (-) sign in case of no amplification.
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