Figure 2
From: Multiplex PCR assay for identification of commonly used disarmed Agrobacterium tumefaciens strains
Multiplex PCR and specificity assessment of C58GlyA-F/R, Ach5FtsZ-F/R, pTiBo542-F/R and nptI-F/R primer pairs for various A. tumefaciens strains. Multiplex PCR using combined primer sets to amplify total genomic DNA of LBA4404, GV3101, C58C1, EHA105 and EHA101 strains respectively (lanes 1–5). All the strains produced specific bands at 61°C and showed no incompatibility or false priming. M is O’RangeRuler™ 100 bp DNA Ladder (Fermentas Lithuania UAB).