Figure 1
From: Multiplex PCR assay for identification of commonly used disarmed Agrobacterium tumefaciens strains
PCR amplification of different A. tumefaciens strains using various primer sets developed in the study. (a) from genomic DNA template of LBA4404 strain using Ach5FtsZ-F/R primer set (Lane 1). (b) from genomic DNA templates of GV3101, C58C1, EHA101 and EHA105 strains respectively using C58GlyA-F/R primer pair (Lanes 2–5). (c) from EHA101 and EHA105 plasmid DNA templates respectively using pTiBo542-F/R primer set. Lane 6, 7). (d) from EHA101 genomic and EHA105 plasmid DNA templates respectively using nptI-F/R primer pair (Lane 8, 9). In all panels first lane marked M represents O’RangeRuler™ 100 bp DNA Ladder (Fermentas Lithuania UAB).