Figure 4

IL17RC induces insensitivity of PI3K, Akt or GSK3. EV-transfected ARPE (A) or THP-1 (B) or IL17RC-transfected ARPE (A) or THP-1 (B) treated without or with sFRP2, cell lysates were immune-blotted with indicated antibodies. In A, GAPDH is the loading control; data represent three or more separate experiments. C. MTT assay of EV-transfected cells or IL17RC- transfected ARPE or THP-1 cells treated without or with sFRP2. Data are averaged from three or more separate experiments and represented as mean ± SD. * or ** versus EV in the cognate groups: p < 0.05 or 0.01. Statistic details of the blots in A or B are shown in Additional file 1: Figure S1C or Additional file 2: Figure S2A.