Figure 1

Microscopic characterization of human breast adipose tissue. A, Normal mammary adipose tissue after Haematoxylin and Eosin staining. Scale bar, 100 μm. B, transmission electron microscopy image of human breast adipose tissue showing perivascular adipose-derived stem cells (ASC) surrounding a small blood vessel with endothelial cells (EC). Scale bar, 10 μm. C, Under fluorescence microscopy, vascular endothelial cells are vonWillenbrand positive (red fluorescence) in the breast adipose tissue, whereas D, adipose-derived stem cell are CD44 positive (green fluorescence). E, Merged image shows that CD44 positive cells reside around endothelial cells of small blood vessels in breast adipose tissue. Nuclei are stained with Hoechst. Scale bar, 100 μm.