Effects of milk-fat globule membrane (MFGM), the phospholipid fraction (PLF), the sphingolipid fraction (SLF), and sphingomyelin (SPM), plus mechanical stretching, on expression of the mRNAs of Dok-7, MuSK, and myogenin in differentiating C2C12 cells. Levels of expression of the mRNAs of Dok-7 (A), MuSK (B), and myogenin (C) were measured by using quantitative real-time PCR. Expression of each gene was normalized against that of the housekeeping gene encoding ribosomal protein, large, P0 (RPLP0/36B4). Values are means ± S.E. of 6 samples. *P < 0.05, significant difference vs. control group by Dunnett’s posthoc test. Values are expressed as ratios, using the value of the control group as 1.0.