Figure 3
From: Cloning, expression and purification of functionally active human angiopoietin-like protein 2
Deglycosylation of Angptl2-GST. Purified Angptl2-GST was incubated with in the absence (Lane A) or presence of PNGase F (Lane B) at 37°C for 5 h and then, along with commercially available human GST-Angptl2 (Novus Biologicals; Catalog number H00023452-P01; Lane C), resolved on SDS-PAGE (10% acrylamide), transferred to nitrocellulose (0.2 μm), and then visualized by immunoblot analysis using an Angptl2-specific antibody.