Figure 1
From: Cloning, expression and purification of functionally active human angiopoietin-like protein 2
Strategy for constructing human Angptl2-GST. A) Map of the SPORT1-hAngptl2 vector obtained from OpenBioSystems. B) PCR amplification of a fragment corresponding to the 3’ region of Angptl2 without the stop codon and subcloning it into the pcDNA3.1 vector. C) Creating a fusion constructs containing the 3’ fragment of Angptl2 plus GST. D) Full-length Angptl2-GST construct (solid arrow) indicating the fragments used in its assembly (open arrows).