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Table 3 PBD with coded levels of ten independent variables and experimental vs. predicted PGase values

From: Raw agro-industrial orange peel waste as a low cost effective inducer for alkaline polygalacturonase production from Bacillus licheniformis SHG10

Trial#

X1

X2

X3

X4

X5

X6

X7

X8

X9

X10

Y (PGase) (U/mL)c

Expa.

Predb.

1

1

-1

1

1

1

1

-1

-1

1

1

0.00

0.1585

2

-1

1

-1

1

1

1

1

-1

-1

1

1.60

1.6135

3

-1

-1

1

-1

1

-1

1

1

1

1

0.64

1.0695

4

1

1

-1

-1

1

1

-1

1

1

-1

0.15

0.0195

5

1

1

1

1

-1

-1

1

1

-1

1

2.70

2.3865

6

-1

-1

-1

1

-1

1

-1

1

1

1

0.00

-0.5795

7

1

-1

1

-1

1

1

1

1

-1

-1

3.04

2.6755

8

-1

1

-1

1

-1

1

1

1

1

-1

0.62

1.0835

9

-1

1

1

-1

-1

-1

-1

1

-1

1

0.00

0.1175

10

-1

-1

-1

-1

1

-1

1

-1

1

1

0.78

0.5935

11

1

1

-1

-1

-1

-1

1

-1

1

-1

1.04

1.0895

12

-1

-1

-1

-1

-1

-1

-1

-1

-1

-1

0.24

-0.0305

13

1

-1

-1

-1

-1

1

-1

1

-1

1

0.00

0.4895

14

-1

1

1

1

1

-1

-1

1

1

-1

0.15

-0.0145

15

1

1

1

-1

-1

1

1

-1

1

1

1.37

1.1425

16

-1

-1

1

1

-1

1

1

-1

-1

-1

1.94

2.0235

17

1

-1

-1

1

1

-1

1

1

-1

-1

2.58

2.6325

18

1

-1

1

1

-1

-1

-1

-1

1

-1

0.00

0.1875

19

-1

1

1

-1

1

1

-1

-1

-1

-1

0.27

0.3635

20

1

1

-1

1

1

-1

-1

-1

-1

1

0.49

0.5885

  1. aExperimental values, bpredicted values, cU/mL: calculated as μg galacturonic acid.min-1.mL-1.
  2. X1: orange peel waste, X2: NaNO3, X3: MgSO4, X4: CaCl2, X5: FeSO4, X6: KNO3, X7: pH, X8: inoculum size, X9: incubation temperature and X10: incubation time.