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Table 1 Comparison of scavenging activities of NS oil and its ME and VO fractions and pure TQ against 2,2-diphenyl-1-picryl hydrazyl (DPPH), hydroxyl (OH), 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and nitric oxide (NO) radicals

From: Mitigating role of thymoquinone rich fractions from Nigella sativa oil and its constituents, thymoquinone and limonene on lipidemic-oxidative injury in rats

Type of free radicals

Test fractions or compounds

 

NS oil

ME

VO

TQ

 

IC50#(μg or mg**/ml)

A. DPPH•

5.12 ± 0.03*

42.14 ± 0.0*

88.39 ± 0.04*

57.15 ± 0.05*

(1.42-10.0)**

(7.14-142.85)

(14.28-342.85)

(8.57-171.42)

B. •OH

14.91 ± 0.01

140.0 ± 0.53

290.0 ± 0.27

186.0 ± 0.5

(5.0-35.0)**

(25.0-500.0)

(50.0-1000.0)

(25.0-500.0)

C. ABTS•+

200.0 ± 0.83

7.81 ± 0.11

17.36 ± 0.17

10.68 ± 0.07

(45.45-545.45)

(1.81-27.27)

(4.54-54.54)

(2.72-22.72)

D. NO•

7.60 ± 0.01

68.75 ± 0.24

140.0 ± 0.22

89.75 ± 0.25

 

(1.0-20.0)**

(5.0-250.0)

(25.0-500.0)

(5.0-250.0)

  1. #IC50 is defined as the concentration (μg or mg**/ml; with range in parenthesis) of test fractions or compounds sufficient to quench or inhibit 50% of the above free radicals (A-D) under standard experimental conditions.
  2. *Values are mean ± SD, of triplicate determinations, were calculated from a concentration dependent plot of each test fraction or compound versus the percent of antioxidant activity.