Figure 6
Effect of methanolic extract (ME) and volatile oil (VO) extracts of Nigella sativa (NS) oil, thymoquinone (TQ) and limonene (LMN) on hepatic lipid hydroperoxide (LOOH) concentration in atherogenic suspension fed rats. NLP-C, normolipidemic control; HLP-C, hyperlipidemic control given one ml of suspension containing 5 mg cholesterol, 30 mg coconut oil and 2.5 mg cholic acid/rat/day; whereas rats in HLP-ME, HLP-VO, HLP-TQ and HLP-LMN were fed one ml of 100 mg ME, 20 mg VO, 10 mg TQ or 200 mg LMN prior to administration of the above atherogenic suspension/rat/day for 30 days of duration. Values are mean (nmol/mg protein) ± SD from homogenates of liver samples in each animal (n = 4 in all groups except n = 5 in NLP-C group). The value of HLP-C control group was statistically significant from NLP-C control group (***p < 0.001). The values of treated groups, HLP-ME, HLP-VO, HLP-TQ and HLP-LMN were significantly different from HLP-C (###p < 0.001) group. The values of treated HLP-VO, HLP-TQ and HLP-LMN groups were significantly different from NLP-C group (θθθp < 0.001), while the value of treated HLP-ME group was not found significantly different from NLP-C group (ns in the figure refers to not significant).