Figure 4
Effect of methanolic extract (ME), volatile oil (VO) fractions, and their constituents, thymoquinone (TQ) and limonene (LMN) on the H 2 O 2 -induced malondialdehyde (MDA) in intact erythrocytes of atherogenic suspension fed rats. NLP-C, normolipidemic control; HLP-C, hyperlipidemic control given one ml of suspension containing 5 mg cholesterol, 30 mg coconut oil and 2.5 mg cholic acid/rat/day; whereas rats in HLP-ME, HLP-VO, HLP-TQ and HLP-LMN were fed one ml of 100 mg ME, 20 mg VO, 10 mg TQ or 200 mg LMN prior to administration of the above atherogenic suspension/rat/day for 30 days of duration. Values are mean ± SD from intact erythrocytes of each animal (n = 4 in all groups except n = 5 in NLP-C group). The value of HLP-C control group was statistically significant from NLP-C control group (***p < 0.001). The values of treated groups, HLP-ME, HLP-VO, HLP-TQ and HLP-LMN were significantly different from HLP-C (###p < 0.001), and NLP-C groups (θθθp < 0.001).