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Figure 4 | SpringerPlus

Figure 4

From: Pcf1, a large subunit of CAF-1, required for maintenance of checkpoint kinase Cds1 activity

Figure 4

Recruitment of Pcf1 to chromatin regulated by the acetylation state of H4. (A) Cell growth of clr6-1 single mutant and clr6-1 Δpcf1 and clr6-1 Δcds1 double mutant cells in the presence or absence of HU. Note the slower growth of the double mutants relative to that of clr6-1 single mutant in the presence of HU. (B) Septation index of clr6-1 single (open square) and clr6-1 Δpcf1 double mutant (closed square) at various times after the HU treatment. (C) Cell growth of wild type and H4.2 K8A K16G mutant cells harboring the empty vector or pREP1-pcf1+ in the presence or absence of thiamine. Note that slow growth by Pcf1 overproduction in wild type cells was suppressed by H4 K8A K16G mutation. (D) Immunoblotting of acetylated H4 (tetra AcH4) from the wild-type and Δpcf1 mutant cells at various times after the HU treatment. As a loading control, an anti-α-tubulin antibody was used. (E) Immunoblotting of chromatin-bound and -unbound fractions prepared from cell extracts of wild-type and H4.2 K8A K16G mutant cells at indicated times after HU treatment.

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