Figure 3

Pcf1-dependent recruitment of Cds1 to chromatin. (A) Immunofluorescent images of antibody-labeled Cds1, DAPI-staining images and their merging images of wild type and Δpcf1 mutant cells. Cells were harvested 6 h after HU treatment and subjected to Triton extraction, followed by staining with the anti-c-myc antibody and DAPI. (B) Mobility of Cds1 from wild type cells harboring the empty vector or pREP-pcf1⁺ in the presence or absence of thiamine (Thi). Note that no mobility shift occurred upon overexpression of pcf1⁺. (C) Immunofluorescent images of antibody-labeled Cds1 after Triton extraction, DAPI-stained images, and their merged images for wild type harboring the empty vector (Vec) or pREP-pcf1⁺ in the absence of thiamine. (D) Incidence of cells with Cds1-myc8-positive nuclei after Triton extraction followed by staining with the anti-c-myc antibody.