DAF-16::GFP does not respond to P. luminescens . (A, B) DAF-16::GFP was located in cytosol when C. elegans was grown on E. coli OP50. (C, D) Cytosolic DAF-16::GFP was diminished when daf-16 was knocked down. (E, F) Cytosolic DAF-16::GFP was accumulated into the cell nuclei when daf-2 was knocked down. (G, H) DAF-16::GFP translocation was not seen when C. elegan s was exposed to P. luminescens TT01. Scale bars, 100 μm.