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Figure 6 | SpringerPlus

Figure 6

From: DLC1 suppresses NF-κB activity in prostate cancer cells due to its stabilizing effect on adherens junctions

Figure 6

DLC1-mediated NF-κB suppression shows effects similar to sh(p65)NF-κB silencing or pharmacological down regulation of NF-κB. (A) Western blot showing NF-κB (p65) knock down, D1, D2, D3 and D4 are different clones. Clone D4 was taken for study. Microphotographs and/or quantitative valuation of Invasion Assay (B) Colony Formation (C) and Apoptosis Assay (D) of C4-2-B2 cells transduced with Lac Z or DLC1, stably transfected with shNF-κB or treated with NF-κB inhibitor (Peptide inhibitor 150 μM). (E) Western blot analysis of NF-κB-induced genes in cell extract from C4-2-B2 transduced and stably transfected with Ad-LacZ + sh-α-catenin, Ad-LacZ + sh-Neo, Ad-DLC1 + sh-α-catenin, Ad-DLC1 + sh-Neo, or NF-κB peptide inhibitor (150 μM).

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