Figure 5
DLC1 expression causes relocation and suppresses phosphorylation and ubiquitination of IκBα. (A) Western blots of phospho-IκBα and total IκBα in Ad-LacZ-, Ad-DLC1-transduced or DLC1 deletion mutant ΔD340-435-transfected C4-2-B2 cells. (B) IκBα localization in Ad-LacZ-, Ad-DLC1-transduced or DLC1 deletion mutant ΔD340-435-transfected C4-2-B2 cells in the presence and absence of calcium. Scale bars 20 μm and 10 μm, respectively. (C) Ubiquitination assay of IκBα of proteasome inhibitor MG132 treated Ad-LacZ and Ad-DLC1 transduced C4-2-B2 and DLC1R718E transduced cells in the presence and absence of calcium.