Figure 2

Both DLC1 and α –catenin expression are required to regulate NF-κB activity. (A) Western blot of total and phosphorylated NF-κB from cell lysates of C4-2-B2 (transduced and/or stably transfected with Ad-LacZ, Ad-DLC1, Ad-DLC1 + sh-Neo or Ad-DLC1 + sh-α-catenin) and PC- 3 cells (transduced with Ad-LacZ, Ad-DLC1 orAd-DLC1 + Ad-α -catenin). (B) Immunostaining of the above described transduced and stable transfected C4-2-B2 and PC- 3 cells with anti-phospho NF-κB, anti-DLC1 and anti-α -catenin antibodies. Scale bar = 20 μm. (C) C4-2-B2, and PC-3 cells were transfected with NF-κB reporter vector and luciferase activity was measured with dual luciferase assay. All values are means ± SD of three independent counts.