Figure 1

DLC1 suppresses NF-κB activation. (A) Relative luciferase activity of NF-κB reporter in RWPE-1, C4-2-B2 and PC-3 cells, (B) Western blot showing the level of DLC1, pNF-κB (p65), NF-κB (p65), RhoAGTP, and total RhoA in RWPE-1, C4-2-B2 and PC-3 cells and level of DLC1 in RWPE-1 and Ad-DLC1 transduced (50MOI) C4-2-B2 and PC-3cells. (C) Western blot showing level of phosphorylated p65 subunit of NF-κB in the Ad-Lac Z and Ad-DLC1 transduced C4-2-B2 and PC-3 cells. (D) Relative luciferase activity of NF-κB reporter in AD-LacZ and DLC1 transduced C4-2-B2 and PC-3 cells. All values are means ± SD of three independent counts. (E) Western blot showing the level of phosphorylated p65 subunit of NF-κB in DLC1- and α-catenin-knock down RWPE-1 cells.