Figure 3

The ubr11-T2 mutant phenocopied ubr11 ∆ mutant for drug resistance. (a) Resistance of the ubr11∆ mutant to anisomycin and hygromycin B (inhibitors of protein synthesis) and terbinafine (an inhibitor of ergosterol synthesis). Serially diluted cells of each strain were spotted on drug-containing or drug-free (control) medium. Note that the strains shown in the second and fifth rows are intrinsically resistant to hygromycin B because they contain the isp4::hphMX6 or ubr11::hphMX6 allele. (b) The host ubr11∆ strain was transformed with plasmids encoding wild type or mutant Ubr11, and the sensitivity of the yeast to the inhibitors was examined as in (a). The ubr11∆ and ubr11-T2 strains showed the same profiles, but the ubr11-T1 profile resembled that of the wild type strain.