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Figure 1 | SpringerPlus

Figure 1

From: Inhibition of neutral sphingomyelinases in skeletal muscle attenuates fatty-acid induced defects in metabolism and stress

Figure 1

Palmitate treatment increases nSMases expression. Differentiated C2C12 myotubes were treated with vehicle control or palmitate or oleate (750 μM) for 16 hrs as described under ‘Methods’ section. After treatments, total RNA was isolated and mRNA levels of nSMase-1 (A), nSMase-2 (B), nSMase-3 (C) and SPTLC1 (D) were quantified by quantitative real time PCR using β-actin as housekeeping gene control. Data are presented as mean + standard deviation. n = 4, **P < 0.01, *P < 0.05, one way ANOVA with Newman-Keuls post test was performed for statistical analyses.

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