Skip to main content
Figure 8 | SpringerPlus

Figure 8

From: Integrated analysis of chronic lipotoxicity on muscle metabolism and stress and its reversal by antioxidants

Figure 8

Scavenging oxidative stress protects myotubes from lipotoxicity. Myotubes were cultured under control or lipotoxic conditions or lipotoxic conditions containing N-acetyl cysteine (NAC). NAC, a ROS scavenger, reduced lipotoxicity induced ROS levels in myotubes (A, *** P < 0.001). (B) Stress signaling as measured by JNK phosphorylation and ER stress as measured by CHOP levels were reduced by NAC. (C) This antioxidant also reduced IMTG levels (*** P < 0.001; ** P < 0.01) and increased mitochondrial DNA copy number (D, * P < 0.05). Expression of PGC1α (E), MEF2C (F) and CPT1β (G) genes was found to be increased by NAC (* P < 0.05, ** P < 0.01, *** P < 0.001). NAC treatment also increased cellular viability (H, *** P < 0.001; * P < 0.05) and ATP levels (I, ** P < 0.01). (J) Lipotoxic conditions decreased and NAC treatment restored SDH activity (*** P < 0.001). (K) Insulin stimulated glucose uptake was decreased under lipotoxic conditions and was restored by NAC treatment (* P < 0.05). ANOVA with Newman-Keuls post test was performed for statistical analyses.

Back to article page