Figure 4
Increased insulin resistance under lipotoxic condition. (A) Post chronic lipotoxic treatment, myotubes were treated for 2 h with or without insulin and gene expression of PDK4 was analyzed (* P < 0.05, *** P < 0.001, ns: not significant). (Con: Control; Lipotox: Lipotoxicity; Ins: Insulin) (B) After chronic lipotoxic treatment, myotubes were serum starved and then treated with or without insulin for 10 min followed by analysis of pAKT and AKT levels by Western blotting. (C) After chronic lipotoxic conditions, myotubes were harvested for p-serine-IRS1 level measurement by Western blotting. Beta actin was used as a loading control.