Figure 2

Lipotoxicity impairs oxidative capacity of myotubes. (A) Fatty acid oxidation ability was measured by HADH activity in lysates of myotubes cultured under control or lipotoxic condition (* P < 0.05). (B) Post lipotoxic condition, mRNA levels of mentioned genes were measured as described in the Methods (* P < 0.05, ** P < 0.01, *** P < 0.001). (C) Cellular NAD levels were quantified after lipotoxic treatment (*** P < 0.001). (D) Total DNA was isolated from myotubes cultured under control or lipotoxic condition and mitochondrial DNA copy number (D) was estimated by assessing mtND1 (mitochondrial gene) copy number normalized to HPRT (nuclear gene) (** P < 0.01). (E) SDH activity in myotubes cultured under control or lipotoxic condition (*** P < 0.001).