Figure 1

Lipotoxic condition augments fat uptake and storage. (A) C2C12 myotubes were treated with lipotoxic condition as described in “Methods” followed by quantitative estimation of triglycerides (*** P < 0.001). Oil-O-red staining showing TG levels in myotubes cultured under lipotoxic condition (C) or under control condition (B). Fatty acid uptake capacity of myotubes (D) was measured by their ability to uptake a fluorescent fatty acid analog after chronic treatment (*** P < 0.001). After lipotoxic condition, RNA was isolated from myotubes and the mRNA levels of FASN, GPAM and LIPIN (E) (* P < 0.05) were measured as described in the Methods.