Figure 3

SDS PAGE analysis for xylanase. (A). SDS-PAGE analysis of B. brevis xylanase expression in two clones (2&3). M: Protein ladder BL: BL21 (DE3) host cell transformed with pET29A vector (Control), 2&3 are two different colonies for pET-29-xylanse transformed in BL21 (DE3). Xylanse protein in dominantly visible only in medium, indicating that most of protein is getting secreted in the growth medium. (B). Xymogram analysis confirms tthat the 23 kDa protein band observed in SDS is a functional Xylanase enzyme.