FGF21 is expressed in brain tissue and by glia cells in culture. (A) Brain regions from adult mice were dissected and subjected to immunoblotting using anti-FGF21 antibodies. β-actin was used as control. FGG21 is present in all brain areas. Two bands are detected representing the precursor and processed, mature form of FGF21 respectively. SN, substantia nigra; ST, striatum; HC, Hippocampus; CX, Cortex. (B) Immunoblot. Glia cell cultures were made from neonatal rat brain as described in Methods. Immunoblotting of cell lysates were performed using anti-FGF21 antibodies. The human hepatocyte cell line (Huh7) was used as a positive control. Note the presence of FGF21 in both cell types.