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Table 5 Primers used in qRT-PCR

From: Comparative analysis reveals similarities between cultured submandibular salivary gland cells and liver progenitor cells

Primer Gene Nucleotide sequence Amplicon size Melting point
bp °C
Control
mGAPDH Glyceraldehyde-3-phosphate dehydrogenase 5′- AGG TCG GTG TGA ACG GAT TTG -3′ 95 62.6
5′- GGG GTC GTT GAT GGC AAC A -3′ 62.6
Hepatic markers
m1AAT Alpha-1-antitrypsin 5′- CTC GTC CGC TCA CTA AAC AAG -3′ 248 60.7
5′- GCT GTC TGA GAG TCA AGG TCT T -3′ 61.3
mAFP Alpha-fetoprotein 5′- CCA TCA CCT TTA CCC AGT TTG T -3′ 101 60.2
5′- CCC ATC GCC AGA GTT TTT CTT -3′ 60.6
mALB Albumin 5′- TGC TTT TTC CAG GGG TGT GTT -3′ 167 62.4
5′- TTA CTT CCT GCA CTA ATT TGG CA -3′ 60.2
mCYP7A1 Cytochrome P450, family 7, subfamily a, polypeptide 1 5′- AAC GGG TTG ATT CCA TAC CTG G -3′ 126 62.0
5′- GTG GAC ATA TTT CCC CAT CAG TT -3′ 60.0
mG6P Glucose-6-phosphatase 5′- CGA CTC GCT ATC TCC AAG TGA -3′ 208 61.0
5′- GGG CGT TGT CCA AAC AGA AT -3′ 60.9
mPEPCK Phosphoenolpyruvate carboxykinase 1 5′- TGA CAG ACT CGC CCT ATG TG -3′ 153 61.0
5′- CCC AGT TGT TGA CCA AAG GC -3′ 61.4
mTAT Tyrosine aminotransferase 5′- AGC CGA ATC CGA ACA AAA CC -3′ 146 60.9
5′- GCC GAT AGA TGG GGC ATA GC -3′ 61.3
mTDO Tryptophan 2,3-dioxygenase 5′- AAT CCA TGA CGA GCA CCT ATT CA -3′ 140 61.4
5′- TCA CCT TGA GCA TGT TCC TCT -3′ 60.8
Liver-enriched transcription factors
mHnf3α Forkhead box A1 (Foxa1) 5′- GGA GTT GAA GTC TCC AGC GTC -3′ 157 62.4
5′- GGG GTG ATT AAA GGA GTA GTG GG -3′ 61.7
mHnf3β Forkhead box A2 (Foxa2) 5′- TCC GAC TGG AGC AGC TAC TAC -3′ 176 62.8
5′- GCG CCC ACA TAG GAT GAC A -3′  
mHhex Hematopoietically expressed homeobox 5′- CGA GAC TCA GAA ATA CCT CTC CC -3′ 162 61.2
5′- CTG TCC AAC GCA TCC TTT TTG -3′ 60.0
mTbx3 T-box 3 (Tbx3), transcript variant 2 5′- TGG AAC CCG AAG AAG ACG TAG -3′ 84 61.2
5′- TAC CCC GCT TGT GAA ACT GG -3′ 62.1
Pancreas-enriched transcription factors
mHes1 Hairy and enhancer of split 1 5′- TCA ACA CGA CAC CGG ACA AAC -3′ 155 63.0
5′- ATG CCG GGA GCT ATC TTT CTT -3′ 61.0
mNgn3 Neurogenin 3 5′- CCA AGA GCG AGT TGG CAC T -3′ 236 62.3
5′- CGG GCC ATA GAA GCT GTG G -3′ 62.5
mPax4 Paired box gene 4 5′- GCA GTG TGA ATC AGC TAG GGG -3′ 103 62.5
5′- CAG GGT CGC ATC CCT CTT ATT -3′ 61.3
mPax6 Paired box gene 6 5′- AAC AGT CAC AGC GGA GTG AAT -3′ 196 61.7
5′- ACA CAA CCG TTG GAT ACG TTT T -3′ 60.7
mPdx1 Pancreatic and duodenal homeobox 1 5′- CCC CAG TTT ACA AGC TCG CT -3′ 117 62.2
5′- CTC GGT TCC ATT CGG GAA AGG -3′ 62.7
mPtf1a Pancreas specific transcription factor, 1a 5′- GCT ACA CGA ATA CTG CTA CCG -3′ 134 60.3
5′- CGC AGC AAT AGC TGA CGT TG -3′ 62.0