Table 5 Primers used in qRT-PCR

Control

mGAPDH

Glyceraldehyde-3-phosphate dehydrogenase

5′- AGG TCG GTG TGA ACG GAT TTG -3′

95

62.6

5′- GGG GTC GTT GAT GGC AAC A -3′

62.6

Hepatic markers

m1AAT

Alpha-1-antitrypsin

5′- CTC GTC CGC TCA CTA AAC AAG -3′

248

60.7

5′- GCT GTC TGA GAG TCA AGG TCT T -3′

61.3

mAFP

Alpha-fetoprotein

5′- CCA TCA CCT TTA CCC AGT TTG T -3′

101

60.2

5′- CCC ATC GCC AGA GTT TTT CTT -3′

60.6

mALB

Albumin

5′- TGC TTT TTC CAG GGG TGT GTT -3′

167

62.4

5′- TTA CTT CCT GCA CTA ATT TGG CA -3′

60.2

mCYP7A1

Cytochrome P450, family 7, subfamily a, polypeptide 1

5′- AAC GGG TTG ATT CCA TAC CTG G -3′

126

62.0

5′- GTG GAC ATA TTT CCC CAT CAG TT -3′

60.0

mG6P

Glucose-6-phosphatase

5′- CGA CTC GCT ATC TCC AAG TGA -3′

208

61.0

5′- GGG CGT TGT CCA AAC AGA AT -3′

60.9

mPEPCK

Phosphoenolpyruvate carboxykinase 1

5′- TGA CAG ACT CGC CCT ATG TG -3′

153

61.0

5′- CCC AGT TGT TGA CCA AAG GC -3′

61.4

mTAT

Tyrosine aminotransferase

5′- AGC CGA ATC CGA ACA AAA CC -3′

146

60.9

5′- GCC GAT AGA TGG GGC ATA GC -3′

61.3

mTDO

Tryptophan 2,3-dioxygenase

5′- AAT CCA TGA CGA GCA CCT ATT CA -3′

140

61.4

5′- TCA CCT TGA GCA TGT TCC TCT -3′

60.8

Liver-enriched transcription factors

mHnf3α

Forkhead box A1 (Foxa1)

5′- GGA GTT GAA GTC TCC AGC GTC -3′

157

62.4

5′- GGG GTG ATT AAA GGA GTA GTG GG -3′

61.7

mHnf3β

Forkhead box A2 (Foxa2)

5′- TCC GAC TGG AGC AGC TAC TAC -3′

176

62.8

5′- GCG CCC ACA TAG GAT GAC A -3′

mHhex

Hematopoietically expressed homeobox

5′- CGA GAC TCA GAA ATA CCT CTC CC -3′

162

61.2

5′- CTG TCC AAC GCA TCC TTT TTG -3′

60.0

mTbx3

T-box 3 (Tbx3), transcript variant 2

5′- TGG AAC CCG AAG AAG ACG TAG -3′

84

61.2

5′- TAC CCC GCT TGT GAA ACT GG -3′

62.1

Pancreas-enriched transcription factors

mHes1

Hairy and enhancer of split 1

5′- TCA ACA CGA CAC CGG ACA AAC -3′

155

63.0

5′- ATG CCG GGA GCT ATC TTT CTT -3′

61.0

mNgn3

Neurogenin 3

5′- CCA AGA GCG AGT TGG CAC T -3′

236

62.3

5′- CGG GCC ATA GAA GCT GTG G -3′

62.5

mPax4

Paired box gene 4

5′- GCA GTG TGA ATC AGC TAG GGG -3′

103

62.5

5′- CAG GGT CGC ATC CCT CTT ATT -3′

61.3

mPax6

Paired box gene 6

5′- AAC AGT CAC AGC GGA GTG AAT -3′

196

61.7

5′- ACA CAA CCG TTG GAT ACG TTT T -3′

60.7

mPdx1

Pancreatic and duodenal homeobox 1

5′- CCC CAG TTT ACA AGC TCG CT -3′

117

62.2

5′- CTC GGT TCC ATT CGG GAA AGG -3′

62.7

mPtf1a

Pancreas specific transcription factor, 1a

5′- GCT ACA CGA ATA CTG CTA CCG -3′

134

60.3

5′- CGC AGC AAT AGC TGA CGT TG -3′

62.0