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Table 1 Antibodies used in the study (asterisks indicate antibodies used for flow cytometry)

From: Comparative analysis reveals similarities between cultured submandibular salivary gland cells and liver progenitor cells

Antibody Manufacturer, catalog number Species Dilution
Primary antibodies
AFP* R&D, # MAB1368 mouse anti-human/mouse IgG1 1:200
ALB* R&D, # MAB1455 mouse anti-human/mouse IgG2a 1:200
CD29* Millipore, # FCMAB269F rat anti-mouse IgG2aκ 2 μl per 106 cells
CD49f* Millipore, # MAB1378 rat anti-human/mouse IgG2a 1:10
CD45* Millipore, # FCMAB126F mouse anti-human/mouse IgG2bκ 10 μl per 106 cells
CD90* Millipore, # CBL1500F mouse anti-rat/mouse IgG1 10 μl per 106 cells
CD133* Millipore, # MAB4310X rat anti- mouse IgG1κ 1:200
CK8 AbCam, # ab59400 rabbit anti-human/rat/mouse IgG 1:500
CK14 Chemicon, # CBL197 mouse anti-human IgG3 1:100
CK18 Millipore, # MAB3234 mouse anti-human/mouse IgG1 1:100
CK19* AbCam, # ab15463-1 rabbit anti-human/ mouse IgG 1:100
CYP P450 1A1 Millipore, # AB1258 rabbit anti-human 1:100
c-Kit* Millipore, # CBL1360 rat anti- mouse IgG2bκ 1 μl per 106 cells
c-Met* Millipore, # MAB3729 mouse anti-human/mouse IgG1 1:500
EpCAM* Abcam, # ab32392 rabbit anti-human/rat/mouse IgG 1:500
Hnf-3β Millipore, # 07-633 rabbit anti-human/rat/mouse IgG 1:200
Hnf-4α Santa Cruz, # SC-8987 rabbit anti-human/rat/mouse IgG 1:200
INS R&D, # MAB1417 rat anti-human/bovine/mouse IgG2A 1:200
Ki67 Millipore, # MAB4190 mouse anti-human IgG1 1:200
NGF Millipore, # 04-1111 rabbit anti-human/rat/mouse IgG 1:200
Sca-1* Millipore, # FCMAB224F rat anti-mouse IgG2aκ 2 μl per 106 cells
Secondary antibodies
Alexa Fluor® 488* Invitrogen, # A-21206 donkey anti-rabbit IgG (H + L) 1:1000
Alexa Fluor® 546 Invitrogen, # A-11035 goat anti-rabbit IgG (H + L) 1:1000
Alexa Fluor® 488* Invitrogen, # A-11029 goat anti-mouse IgG (H + L) 1:1000
Alexa Fluor® 488* Invitrogen, # A-11006 goat anti-rat IgG (H + L) 1:1000