Comparative analysis reveals similarities between cultured submandibular salivary gland cells and liver progenitor cells

Petrakova, Olga S; Terskikh, Vasiliy V; Chernioglo, Elena S; Ashapkin, Vasiliy V; Bragin, Evgeny Y; Shtratnikova, Victoria Y; Gvazava, Inessa G; Sukhanov, Yuriy V; Vasiliev, Andrey V

doi:10.1186/2193-1801-3-183

SpringerPlus

Table 1 Antibodies used in the study (asterisks indicate antibodies used for flow cytometry)

From: Comparative analysis reveals similarities between cultured submandibular salivary gland cells and liver progenitor cells

Antibody	Manufacturer, catalog number	Species	Dilution
Primary antibodies
AFP*	R&D, # MAB1368	mouse anti-human/mouse IgG1	1:200
ALB*	R&D, # MAB1455	mouse anti-human/mouse IgG2a	1:200
CD29*	Millipore, # FCMAB269F	rat anti-mouse IgG2aκ	2 μl per 10⁶ cells
CD49f*	Millipore, # MAB1378	rat anti-human/mouse IgG2a	1:10
CD45*	Millipore, # FCMAB126F	mouse anti-human/mouse IgG2bκ	10 μl per 10⁶ cells
CD90*	Millipore, # CBL1500F	mouse anti-rat/mouse IgG1	10 μl per 10⁶ cells
CD133*	Millipore, # MAB4310X	rat anti- mouse IgG1κ	1:200
CK8	AbCam, # ab59400	rabbit anti-human/rat/mouse IgG	1:500
CK14	Chemicon, # CBL197	mouse anti-human IgG3	1:100
CK18	Millipore, # MAB3234	mouse anti-human/mouse IgG1	1:100
CK19*	AbCam, # ab15463-1	rabbit anti-human/ mouse IgG	1:100
CYP P450 1A1	Millipore, # AB1258	rabbit anti-human	1:100
c-Kit*	Millipore, # CBL1360	rat anti- mouse IgG2bκ	1 μl per 10⁶ cells
c-Met*	Millipore, # MAB3729	mouse anti-human/mouse IgG1	1:500
EpCAM*	Abcam, # ab32392	rabbit anti-human/rat/mouse IgG	1:500
Hnf-3β	Millipore, # 07-633	rabbit anti-human/rat/mouse IgG	1:200
Hnf-4α	Santa Cruz, # SC-8987	rabbit anti-human/rat/mouse IgG	1:200
INS	R&D, # MAB1417	rat anti-human/bovine/mouse IgG2A	1:200
Ki67	Millipore, # MAB4190	mouse anti-human IgG1	1:200
NGF	Millipore, # 04-1111	rabbit anti-human/rat/mouse IgG	1:200
Sca-1*	Millipore, # FCMAB224F	rat anti-mouse IgG2aκ	2 μl per 10⁶ cells
Secondary antibodies
Alexa Fluor® 488*	Invitrogen, # A-21206	donkey anti-rabbit IgG (H + L)	1:1000
Alexa Fluor® 546	Invitrogen, # A-11035	goat anti-rabbit IgG (H + L)	1:1000
Alexa Fluor® 488*	Invitrogen, # A-11029	goat anti-mouse IgG (H + L)	1:1000
Alexa Fluor® 488*	Invitrogen, # A-11006	goat anti-rat IgG (H + L)	1:1000

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