|
Primary antibodies
|
|
AFP*
|
R&D, # MAB1368
|
mouse anti-human/mouse IgG1
|
1:200
|
|
ALB*
|
R&D, # MAB1455
|
mouse anti-human/mouse IgG2a
|
1:200
|
|
CD29*
|
Millipore, # FCMAB269F
|
rat anti-mouse IgG2aκ
|
2 μl per 106 cells
|
|
CD49f*
|
Millipore, # MAB1378
|
rat anti-human/mouse IgG2a
|
1:10
|
|
CD45*
|
Millipore, # FCMAB126F
|
mouse anti-human/mouse IgG2bκ
|
10 μl per 106 cells
|
|
CD90*
|
Millipore, # CBL1500F
|
mouse anti-rat/mouse IgG1
|
10 μl per 106 cells
|
|
CD133*
|
Millipore, # MAB4310X
|
rat anti- mouse IgG1κ
|
1:200
|
|
CK8
|
AbCam, # ab59400
|
rabbit anti-human/rat/mouse IgG
|
1:500
|
|
CK14
|
Chemicon, # CBL197
|
mouse anti-human IgG3
|
1:100
|
|
CK18
|
Millipore, # MAB3234
|
mouse anti-human/mouse IgG1
|
1:100
|
|
CK19*
|
AbCam, # ab15463-1
|
rabbit anti-human/ mouse IgG
|
1:100
|
|
CYP P450 1A1
|
Millipore, # AB1258
|
rabbit anti-human
|
1:100
|
|
c-Kit*
|
Millipore, # CBL1360
|
rat anti- mouse IgG2bκ
|
1 μl per 106 cells
|
|
c-Met*
|
Millipore, # MAB3729
|
mouse anti-human/mouse IgG1
|
1:500
|
|
EpCAM*
|
Abcam, # ab32392
|
rabbit anti-human/rat/mouse IgG
|
1:500
|
|
Hnf-3β
|
Millipore, # 07-633
|
rabbit anti-human/rat/mouse IgG
|
1:200
|
|
Hnf-4α
|
Santa Cruz, # SC-8987
|
rabbit anti-human/rat/mouse IgG
|
1:200
|
|
INS
|
R&D, # MAB1417
|
rat anti-human/bovine/mouse IgG2A
|
1:200
|
|
Ki67
|
Millipore, # MAB4190
|
mouse anti-human IgG1
|
1:200
|
|
NGF
|
Millipore, # 04-1111
|
rabbit anti-human/rat/mouse IgG
|
1:200
|
|
Sca-1*
|
Millipore, # FCMAB224F
|
rat anti-mouse IgG2aκ
|
2 μl per 106 cells
|
|
Secondary antibodies
|
|
Alexa Fluor® 488*
|
Invitrogen, # A-21206
|
donkey anti-rabbit IgG (H + L)
|
1:1000
|
|
Alexa Fluor® 546
|
Invitrogen, # A-11035
|
goat anti-rabbit IgG (H + L)
|
1:1000
|
|
Alexa Fluor® 488*
|
Invitrogen, # A-11029
|
goat anti-mouse IgG (H + L)
|
1:1000
|
|
Alexa Fluor® 488*
|
Invitrogen, # A-11006
|
goat anti-rat IgG (H + L)
|
1:1000
|
