Figure 5

Immunocytochemical analysis of EpCAM expression features in first-passage SGC culture. Cell nuclei were stained with DAPI (blue), EpCAM was stained with Alexa Fluor 488-conjugated antibodies (green), and alpha-fetoprotein was stained with Alexa Fluor 546-conjugated antibodies (red); scale bars = 100 μm: (A) membrane localization of EpCAM (paraformaldehyde fixation without Triton X-100); (B) cytoplasmic localization of EpCAM (fixation with 70% ethanol); (C) alpha-fetoprotein; (D) EpCAM; (E) coexpression of alpha-fetoprotein and EpCAM.