Figure 9
Amyloid-β peptides in Tg+ve neurosphere culture supernatants contain Aβ40 and Aβ42 peptides. (A) Sixty microgram of total protein from concentrated neurosphere culture supernatants were size fractionated in 10% Tris-Bicine-Urea-SDS-PAGE along with 5 ng of monomeric Aβ40 and Aβ42 peptides, followed by immunoblotting with 6E10 antibody after HIER. Tg+ve NS1 and NS3 neurosphere culture supernatants contain both Aβ40 and Aβ42 peptides but they are not seen in Tg-ve neurosphere culture supernatants. (B) Images from a shorter exposure show large oligomeric Aβ levels are higher in NS1 than NS3 culture supernatants. (C) Densitometric analysis of Aβ40 and Aβ42 peptide bands indicates the ratio of Aβ42/Aβ40 in NS1 and NS3 culture supernatants as 0.875 ± 0.336 and 0.989 ± 0.487 respectively. (D) Densitometric analysis of large Aβ oligomers is presented as histogram of mean + standard deviation from four separate experiments.