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Figure 4

From: Development of a novel cellular model of Alzheimer’s disease utilizing neurosphere cultures derived from B6C3-Tg(APPswe,PSEN1dE9)85Dbo/J embryonic mouse brain

Figure 4

Transgene positive neurosphere cultures transcribe both the transgenes, express humanized APP and co-express nestin. (A) Detection of huAPPswe (233-bp) and huPSEN1dE9 (141-bp) transcripts in neurosphere lines by RT-PCR analysis. A 599-bp GAPDH fragment was amplified to validate equal RNA loading in all NS lines. (B) Immunofluorocytochemistry of nestin shows the majority of cells in all neurosphere lines express nestin. (C) Fluorescence images show expression of huAPPswe protein only in Tg+ve neurospheres using 6E10 antibody. Nuclei were stained with DAPI. (D) Fluorescence intensity from individual cells was derived and intensity above secondary antibody signal was considered as positive. Percentage of cells positive for each antibody from three independent experiments is demonstrated as histograms of mean ± standard deviation.

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