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Figure 3

From: Development of a novel cellular model of Alzheimer’s disease utilizing neurosphere cultures derived from B6C3-Tg(APPswe,PSEN1dE9)85Dbo/J embryonic mouse brain

Figure 3

Expression of nestin and β-tubulin III in NS cultures. (A) Monolayer of cells was cultured as described earlier. Immunostaining with nestin and β-tubulin III antibodies, image acquisition, analysis and display are similar to Figure 2. Images demonstrate that some cells are positive for both nestin and β-tubulin III (yellow arrowheads). (B) Percent cells positive either for nestin, β-tubulin III or for both (co-stain) was plotted as histograms of mean + standard deviation from 6 images from two independent data sets. Significant increase in nestin and β-tubulin III co-stain cells is observed in NS3 line. *indicates p ≤ 0.05 when compared with other neurospheres and **indicates p ≤ 0.05 when compared with NS1 co-stain cells.

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