Figure 2

Expression of nestin and glial fibrillary acidic protein (GFAP) in neurosphere cultures. (A) Fixed monolayer culture of neurosphere cultures, cells were immunostained with anti-nestin and anti-GFAP antibodies (for details see materials and methods). All the images are displayed at the same intensity scale. Images show some nestin positive cells are also positive for GFAP (yellow arrowhead). (B) Cell type analysis was performed by counting the cells positive for each marker manually, using ImageJ 1.42q software (NIH). Percent cells positive either for nestin, GFAP or for both markers (co-stain) was plotted as histograms of mean + standard deviation of three independent experiments. No significant difference is found between GFAP stained cell populations among NS1, NS2, NS3 and NS4 lines regardless of the presence of transgenes.